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ladders  (Bio-Rad)


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    Structured Review

    Bio-Rad ladders
    Ladders, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1074 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+ladder/Precision+Plus+Protein+All+Blue+Prestained+Protein+Standards/pmc13091216-163-1-12
    Average 96 stars, based on 1074 article reviews
    ladders - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: RNAi-mediated p38δ silencing mitigates anthracycline cardiotoxicity in female mice.
    Article Snippet: 313 Proteins were separated by molecular weight through electrophoresis using a 4-15% Criterion 314 TGX gel (Bio-Rad, 5671084) and then transferred to a PVDF membrane. .. The membrane was 315 blocked with bovine serum albumin and horizontally cut into two pieces according to the 316 molecular weight of the protein ladder (Bio-Rad, 1610375) for separate detection of 317 housekeeping and target proteins. ..

    Molecular Weight:

    Article Title: RNAi-mediated p38δ silencing mitigates anthracycline cardiotoxicity in female mice.
    Article Snippet: 313 Proteins were separated by molecular weight through electrophoresis using a 4-15% Criterion 314 TGX gel (Bio-Rad, 5671084) and then transferred to a PVDF membrane. .. The membrane was 315 blocked with bovine serum albumin and horizontally cut into two pieces according to the 316 molecular weight of the protein ladder (Bio-Rad, 1610375) for separate detection of 317 housekeeping and target proteins. ..

    other:

    Article Title: Pervasive Cas9 expression driven by mammalian promoter activity in E. coli affects representation of CRISPR sgRNA libraries.
    Article Snippet: Transformation of NEB Stable Competent E. coli (High Efficiency) (NEB, catalogue number C3040H) was performed according to manufacturer instructions using 2uL of DNA per transformation.

    Staining:

    Article Title: Diving into the unknown: Evidence of enhanced skeletal muscle lactate efflux potential and blood–muscle exchange in competitive breath‐hold divers
    Article Snippet: Working samples of equal concentration were prepared by dilutingmuscle homogenates in Laemmli buffer (Bio-Rad, Hercules, CA, USA), β-mercaptoethanol (M6250, Sigma Aldrich, Toronto, ON, Canada) and RIPA buffer, followed by heating at 95°C for 5 min. .. Subsequently equal amounts of protein were loaded onto 4%–15% Criterion TGX Stain-Free protein gels (Bio-Rad) and electrophoresed at 200 V for 45 min. All gels were run with a protein ladder (Precision Plus Protein All Blue Protein Standard, Bio-Rad). .. Proteins were then wet-transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad).

    Incubation:

    Article Title: Müllerian inhibiting substance type 2 receptor (MISIIR)-specific CAR T cells for the treatment of ovarian cancer and other gynecologic malignancies
    Article Snippet: Protein was quantified using a bicinchoninic acid (BCA) assay (Thermo Scientific, Waltham, MA, USA; catalog no. 23227). .. 80 μg of protein samples was mixed with Laemmli loading buffer (Bio-Rad, Hercules, CA, USA; catalog no. 161-0737) and 5% β-mercaptoethanol (Bio-Rad, Hercules, CA, USA; catalog no. 60-24-2) and incubated at 95° C. for 5 min. Lysates were loaded in 4%-15% Mini-PROTEAN TGX gels (Bio-Rad, Hercules, CA, USA; catalog no. 456-1033), and gels were run at 150 V for 1 h. A protein ladder (Bio-Rad, Hercules, CA, USA; catalog no. 161-0376) was used. .. Protein samples were then transferred from gels to a polyvinylidene fluoride (PVDF) membrane (Millipore, Burlington, MA, USA; catalog no. IPVH00010) at 100 V for 1 h. Membranes were blocked and washed with Tris-buffered saline with Tween 20 (TBST) (1% Tween 20) (Bio-Rad, Hercules, CA, USA; catalog no. 170-6435) and incubated with primary human MISIIR antibody (R&D Systems, Minneapolis, MN, USA; catalog no. AF4749) at 0.1 mg/mL and secondary antibody peroxidase-conjugated AffiniPure donkey anti-sheep IgG (H+L) (Jackson ImmunoResearch, West Grove, PA, USA; catalog no. 713-035-147) diluted 1:10,000.

    Article Title: PEPITEM Regulates the Synovial Microenvironment During Immune-Mediated Inflammatory Arthritis to Limit Disease.
    Article Snippet: Protein quantification was performed using the Pierce BSA Protein Assay (cat: 23225; Thermo Fisher Scientific). .. PBMC cell lysates (10 μg) or protein ladder (5 μL) were diluted 1:1 with 2x Laemmli sample buffer (Bio-Rad) and incubated at 95 C for five minutes. .. Samples were loaded onto a 10% Criterion Tris-HCL protein gel (cat: 3450009; Bio-Rad) and electrophoresed at 100 V for 90 minutes.



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